The Yeast Intein Database

The Yeast Intein Database is a comprehensive and curated database devoted to yeast inteins.


Inteins are self-catalytic protein splicing elements. The Yeast Intein Database and Registry, is a curated compilation of published and unpublished information about protein splicing. It presents general information as well as detailed data for each yeast intein, including tabulated comparisons and a comprehensive bibliography. An intein-specific BLAST server is now available to assist in identifying new inteins.

 

Intein Name Spa VMA-2
Intein id YSTIN075
Intein Class Theoretical
Prototype Allele Sce VMA
Organism Name Saccharomyces pastorianus, strain KBY001
Exiein Name Vacuolar ATPase (H+-transporting ATP synthase), subunit A
Intein Class Theoretical
Organism Description Yeast, taxon:27292
Domain of Life Eucarya
Endonuclease Activity --
Endonuclease Motif DOD
Location in Extein G269
Inert Site Comment VMA-a
Intein Size 586
Intein N Terminal -
Intein C Terminal KS/CRGCCVGEQL
Accession No BAA94596.1 in NCBI/protein
Intein aa Sequence >gi|7576436|dbj|BAA94596.1| endonuclease for mating-type conversion [Saccharomyces pastorianus] MLSENTTILMANGEIKDIANVTANSYVMCEDGSAARVISVTQGCQKIYNIQQKTKHRAFEGEPGRLDPRR RTIYQRLDLQCTAGHKLSVRVPTKPLLEKSGRSATKYKVRWRNLQQCQTLDGRIITIPKNHHKTFPMTVE GEFAAKCFIEEMELLKGEYFNFDIEVRDLDYLDAQLRISSCIRFSPVIAGNGVLSKFLTGRNDLVTPAVK SMAWMLGLWLGDGTTKEPEISVDSLDPKLMESLREQAKIWGLYLTVCDDHVPLRAKHVRLHYGDGPDENR KTKNLRKNNPFWNAVTKLKFKRELDGEKQIPEFMYSEHVEVREAFLAGLIDSDGYVVKKGEGPESYKIAI QTVYSSIMDGVVHISRSLGMSATVTTRSAREEIIEGRKVQCQFTYDCNVAGGTTLQNVLSYCRSGHKTRE IPPIVKREPVYFGFTDDFQGESTVYGLHIEGHKSYLLGNKIEVKS
Block A MLSENTTILMANG 13
Block B RLDLQCTAGHKLSV 89
Block C MLGLWLGDG 223
Block D EKQIPEFM 314
Block E FLAGLIDSDG 334
Block H TVYSSIMDGVVHISRSLGM 370
Block F TVYGHHIEGHKSYL 456
Block G NKIEVKS 465
Contributed by Dhiraj Sharma, Neelmani Vashistha, Surender Yadav
Comments This intein is 80% similar to Spa VMA, Sce-OUT7112 VMA, Sce-DH1-1A VMA and having N-terminal and C- terminal motifs and similar four endonuclease motifs.
References --

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