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| The
Yeast Intein Database |
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The Yeast Intein Database is a comprehensive
and curated database devoted to yeast inteins.
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| Inteins
are self-catalytic protein splicing elements. The Yeast Intein Database
and Registry, is a curated compilation of published and unpublished
information about protein splicing. It presents general information
as well as detailed data for each yeast intein, including tabulated
comparisons and a comprehensive bibliography. An intein-specific BLAST
server is now available to assist in identifying new inteins. |
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| Intein
Name |
Sce VMA-5
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| Intein
id |
YSTIN064 |
| Intein
Class |
Theoretical
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| Prototype
Allele |
Sce VMA
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| Organism
Name |
Saccharomyces cerevisiae, strain S288C |
| Exiein
Name |
Vacuolar ATPase (H+-transporting ATP synthase), subunit A |
| Intein
Class |
Theoretical |
| Organism
Description |
Yeast,taxon:4932 |
| Domain
of Life |
Eucarya |
| Endonuclease
Activity |
-- |
| Endonuclease
Motif |
DOD |
| Location
in Extein |
G269 |
| Inert
Site Comment |
VMA-a |
| Intein
Size |
586 |
| Intein
N Terminal |
-------------------- |
| Intein
C Terminal |
KS/CGNNAGARIC |
| Accession
No |
NP_010054.1 in NCBI/protein, |
| Intein
aa Sequence |
>gi|6319974|ref|NP_010054.1| Site-specific endonuclease required for gene conversion at the MAT locus (homothallic switching) through the generation of a ds DNA break; expression restricted to mother cells in late G1 as controlled by Swi4p-Swi6p, Swi5p and Ash1p; Hop [Saccharomyces cerevisiae]
MLSENTTILMANGEIKDIANVTANSYVMCADGSAARVINVTQGYQKIYNIQQKTKHRAFEGEPGRLDPRR
RTVYQRLALQCTAGHKLSVRVPTKPLLEKSGRNATKYKVRWRNLQQCQTLDGRIIIIPKNHHKTFPMTVE
GEFAAKRFIEEMERSKGEYFNFDIEVRDLDYLDAQLRISSCIRFGPVLAGNGVLSKFLTGRSDLVTPAVK
SMAWMLGLWLGDSTTKEPEISVDSLDPKLMESLRENAKIWGLYLTVCDDHVPLRAKHVRLHYGDGPDENR
KTRNLRKNNPFWKAVTILKFKRDLDGEKQIPEFMYGEHIEVREAFLAGLIDSDGYVVKKGEGPESYKIAI
QTVYSSIMDGIVHISRSLGMSATVTTRSAREEIIEGRKVQCQFTYDCNVAGGTTSQNVLSYCRSGHKTRE
VPPIIKREPVYFSFTDDFQGESTVYGLTIEGHKNFLLGNKIEVKSCRGCCVGEQLKISQKKNLKHCVACP
RKGIKYFYKDWSGKNRVCARCYGRYKFSGHHCINCKYVPEAREVKKAKDKGEKLGITPEGLPVKGPECIK
CGGILQFDAVRGPHKSCGNNAGARIC |
| Block
A |
MLSENTTILMANG 13 |
| Block
B |
RLALQCTAGHKLSV 89 |
| Block
C |
MLGLWLGDG 223 |
| Block
D |
EKQIPEFM 314 |
| Block
E |
FLAGLIDSDG 334
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| Block
H |
TVYSSIMDGIVHISRSLGM 370 |
| Block
F |
YGLTIEG**--HKNFL 456 |
| Block
G |
NKIEVKS 465
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| Contributed
by |
Dhiraj Sharma , Neelmani Vashistha , Suernder Yadav |
| Comments |
This intein is 80% similar to Sce VMA, Sce-OUT7091 VMA, Spa VMA, Sce-OUT7112 VMA , Sce-DH1-1A VMA, Scar VMA intein and having similar N –terminal and C -terminal motifs and similar four endonuclease motifs. |
| References |
Okuda 2003 |
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Please
send comments and suggestions to curator at: curator@ibibiosolutions.com
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Developed
at the Bioinformatics Research Laboratory
of IBI Biosolutions Pvt. Ltd.
Copyright
© IBI Biosolutions Pvt. Ltd.. (2008).
For problems or questions regarding this Web site contact us at: curator@ibibiosolutions.com
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IBI Databases and associated information are protected by copyright.
This server and its associated data and services are for academic, non-commercial
use only. The IBI has no liability for the use of results, data or information
which have been provided through this server. Neither the use for commercial
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nor the distribution of parts of files or derivative products to any
third parties is permitted. Commercial users may contact the IBI BIosolutions
Pvt. Ltd.
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