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| The
Yeast Intein Database |
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The Yeast Intein Database is a comprehensive
and curated database devoted to yeast inteins.
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| Inteins
are self-catalytic protein splicing elements. The Yeast Intein Database
and Registry, is a curated compilation of published and unpublished
information about protein splicing. It presents general information
as well as detailed data for each yeast intein, including tabulated
comparisons and a comprehensive bibliography. An intein-specific BLAST
server is now available to assist in identifying new inteins. |
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| Intein
Name |
Sce VMA-2
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| Intein
id |
YSTIN061 |
| Intein
Class |
Theoretical
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| Prototype
Allele |
Sce VMA
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| Organism
Name |
Saccharomyces cerevisiae , strain OUT7009 |
| Exiein
Name |
Vacuolar ATPase (H+-transporting ATP synthase), subunit A |
| Intein
Class |
Theoretical |
| Organism
Description |
Yeast,taxon :4932 |
| Domain
of Life |
Eucarya |
| Endonuclease
Activity |
PI-SceI |
| Endonuclease
Motif |
DOD |
| Location
in Extein |
G283 |
| Inert
Site Comment |
VMA-a |
| Intein
Size |
454 |
| Intein
N Terminal |
SNSDAIIYVG/C |
| Intein
C Terminal |
HN/CGERGNEMAE |
| Accession
No |
BAC66638.1 in NCBI/protein, |
| Intein
aa Sequence |
>gi|29420833|dbj|BAC66638.1| vacuolar membrane ATPase subunit a precursor [Saccharomyces cerevisiae]
CFAKGTNVLMADGSIECIENIE
VGNKVMGKDGRPREVIKLPRGRETMYSVVQKSQHRAHKSDSSREVPELLKFTCNATHELVVRTPRSVRRL
SRTIKGVEYFEVITFEMGQKKAPDGRIVELVKEVSKSYPISEGPERANELVESYRKASNKAYFEWTIEAR
DLSLLGSHVRKATYQTYAPILYENDHFFDYMQKSKFHLTIEGPKVLAYLLGLWIGDGLSDRATFSVDSRD
TSLMERVTEYAEKLNLCAEYKDRKEPQVAKTVNLYSKVVRGNGIPNNLNTENPLWDAIVGLGFLKDGVKN
IPSFLSTDNIGTRETFLAGLIDSDGYVTDEHGIKATIKTIHTSVRDGLVSLARSLGLVVSVNAEPAKVDM
NGTKHKISYAIYMSGGDVLLNVLSKCAGSKKFRPAPAAAFARECRGFYFELQELKEDDYYGITLSDDSDH
QFLLANQVVVHN |
| Block
A |
CFAKGTNVLMADG 13 |
| Block
B |
LLKFTCNATHELVV 83 |
| Block
C |
LLGLWIGDG 219 |
| Block
D |
VKNIPSFL 307 |
| Block
E |
FLAGLIDSDG 327
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| Block
H |
TIHTSVRDGLVSLARSLGL 359 |
| Block
F |
YGITLS**DDSDHQFL 445 |
| Block
G |
NQVVVHN 454
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| Contributed
by |
Dhiraj Sharma , Neelmani Vashistha , Suernder Yadav |
| Comments |
This intein is 99% similar to Sce VMA, Sce-OUT7091 VMA, Spa VMA, Sce-OUT7112 VMA , Sce-DH1-1A VMA, Scar VMA intein and having similar N –terminal and C -terminal motifs and similar four endonuclease motifs. |
| References |
Okuda 2003 |
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Please
send comments and suggestions to curator at: curator@ibibiosolutions.com
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of IBI Biosolutions Pvt. Ltd.
Copyright
© IBI Biosolutions Pvt. Ltd.. (2008).
For problems or questions regarding this Web site contact us at: curator@ibibiosolutions.com
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