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| The
Yeast Intein Database |
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The Yeast Intein Database is a comprehensive
and curated database devoted to yeast inteins.
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| Inteins
are self-catalytic protein splicing elements. The Yeast Intein Database
and Registry, is a curated compilation of published and unpublished
information about protein splicing. It presents general information
as well as detailed data for each yeast intein, including tabulated
comparisons and a comprehensive bibliography. An intein-specific BLAST
server is now available to assist in identifying new inteins. |
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| Intein
Name |
Sce VMA
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| Intein
id |
YSTIN025 |
| Intein
Class |
Experimental
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| Prototype
Allele |
Sce VMA
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| Organism
Name |
Saccharomyces cerevisiae (nucleus) |
| Exiein
Name |
Vacuolar ATPase (H+-transporting ATP synthase), subunit A |
| Intein
Class |
Experimental |
| Organism
Description |
Yeast, also in Sce strains OUT7163, OUT7045, OUT7163, IFO1992 |
| Domain
of Life |
Eucarya |
| Endonuclease
Activity |
PI-SceI |
| Endonuclease
Motif |
DOD |
| Location
in Extein |
G283 |
| Inert
Site Comment |
VMA-a |
| Intein
Size |
454 |
| Intein
N Terminal |
SNSDAIIYVG/C |
| Intein
C Terminal |
HN/CGERGNEMAE |
| Accession
No |
PXBYVA in NCBI/protein |
| Intein
aa Sequence |
>gi|67951|pir||PXBYVA Sce VMA intein (including -1 and +1 extein residues)
GCFAKGTNVLMADGSIECIENIEVGNKVMGKDGRPREVIKLPRGRETMYSVVQKSQHRAHKSDSSREVPE
LLKFTCNATHELVVRTPRSVRRLSRTIKGVEYFEVITFEMGQKKAPDGRIVELVKEVSKSYPISEGPERA
NELVESYRKASNKAYFEWTIEARDLSLLGSHVRKATYQTYAPILYENDHFFDYMQKSKFHLTIEGPKVLA
YLLGLWIGDGLSDRATFSVDSRDTSLMERVTEYAEKLNLCAEYKDRKEPQVAKTVNLYSKVVRGNGIRNN
LNTENPLWDAIVGLGFLKDGVKNIPSFLSTDNIGTRETFLAGLIDSDGYVTDEHGIKATIKTIHTSVRDG
LVSLARSLGLVVSVNAEPAKVDMNGTKHKISYAIYMSGGDVLLNVLSKCAGSKKFRPAPAAAFARECRGF
YFELQELKEDDYYGITLSDDSDHQFLLANQVVVHNC |
| Block
A |
CFAKGTNVLMADG 13 |
| Block
B |
LLKFTCNATHELVV 83 |
| Block
C |
LLGLWIGDG 219 |
| Block
D |
VKNIPSFL 307 |
| Block
E |
FLAGLIDSDG 327
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| Block
H |
TIHTSVRDGLVSLARSLGL 359 |
| Block
F |
YGITLS**DDSDHQFL 445 |
| Block
G |
NQVVVHNC 455
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| Contributed
by |
Yasuhiro Anraku |
| Comments |
Sce VMA is a nuclear gene. An identical intein aa sequence is present in strain Out7163, (BAC66646.1), OUT7045 (BAC6664), OUT7163 (BAC66646),IFO1992 (BAC66650). Also see AAA34664 NCBI/protein |
| References |
Shih 1988
Hirata 1990
Kane 1990
Hirata 1992
Bremer 1992
Ryan 1992
Gimble 1992
Cooper 1993
Gimble 1993
Anraku 1994
Gimble 1995
Gimble 1996
Chong 1996
Kawasaki 1996
Wende 1996
Chong 1997
Chong 1997B
Duan 1997
Kawasaki 1997
Kawasaki 1997B
Nogami 1997
He 1998
Chong 1998
Grindl 1998
Muir 1998
Pingoud 1998
Severinov 1998
Chong 1998B
Cotton 1999
Xu 1999
Yu 1999
Pingoud 1999
Hu 1999
Miyamoto 1999
Hu 2000
Poland 2000
Gimble 2001
Mizutani 2002
Posey 2002
Morassutti 2002
Moure 2002
Werner 2002
Nogami 2002
Okuda 2003
Fukuda 2003
Gimble 2003
Steuer 2004
Mizutani 2004
Anraku 2005 |
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send comments and suggestions to curator at: curator@ibibiosolutions.com
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Developed
at the Bioinformatics Research Laboratory
of IBI Biosolutions Pvt. Ltd.
Copyright
© IBI Biosolutions Pvt. Ltd.. (2008).
For problems or questions regarding this Web site contact us at: curator@ibibiosolutions.com
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