The Yeast Intein Database

The Yeast Intein Database is a comprehensive and curated database devoted to yeast inteins.


Inteins are self-catalytic protein splicing elements. The Yeast Intein Database and Registry, is a curated compilation of published and unpublished information about protein splicing. It presents general information as well as detailed data for each yeast intein, including tabulated comparisons and a comprehensive bibliography. An intein-specific BLAST server is now available to assist in identifying new inteins.

 

Intein Name Pgu-alt GLT1
Intein id YSTIN020
Intein Class Theoretical
Prototype Allele Dhan GLT1
Organism Name Pichia (Candida) guilliermondii
Exiein Name Glutamate synthase, alt. code 12
Intein Class Theoretical
Organism Description Fungi
Domain of Life Eucarya
Endonuclease Activity --
Endonuclease Motif DOD
Location in Extein K?
Inert Site Comment GLT1-a, R1183 of Dha GluSyn
Intein Size 553
Intein N Terminal IAMGCIMMRK/C
Intein C Terminal SN/CHLNTCPVGI
Accession No http://www.broad.mit.edu/annotation/fungi/fgi
Intein aa Sequence >Broad Instidute genome sequencing project | Pgu-alt GLT1 intein (including -1 and +1 extein residues) KCLASDTLVRTTQGLKKVKDVSVGEYLYGADNTPVLCIGANAPETGSLKEIVYEDFDSKKLVSFKCTPDHHLELVLANAA PELSGSTVTWFSSCTGEHKKQDSTFDLESIVSSFYNDLVDSDDIITFDNVCEAIDVALDEHYHRGGSDRYSEKFNGFITQ IANEELKSNPEFVRNALHEAAEKFMVEDAPKNFAVTSVSVETTSVESQSVCKSSCACGGVRKIVHQFETIEEAEMAYSLL LSDVYDRVDPSFVSNGYKFQASVEALEQMCSKDILHNHLHMYRAPAEQVEPLTHSSSLPVDPYFLGLWLGEGSSDSATIS TTDREVVVWLQQYVERLNKDKPAEASPLRLTKINGFKPGQQMANGFVQKVTVPTYTITSGESFTGGNWNPVYNSLKSMGL LSNKSNGIPEAYKAADLQSRLALLAGLVDSDGCYVESYNSYRFMQKSELHKKIVYDLKEMATSCGIETTDIDVSESRLYL GSSETTSAYVVYLGSGSEKLQQSLLLPRKKMVRIESCIQESTPFKVQDVANGEFRAIEVSGGKFQLDNGLQIANC
Block A CLASDTLVRTTQG 13
Block B LVSFKCTPDHHLEL 73
Block C FLGLWLGEG 311
Block D NKDKPAEA 344
Block E LLAGLVDSDG 431
Block H ELHKKIVYDLKEMATSCGI 465
Block F GEFRAI**EVSGGKFQ 544
Block G NGLQIANC 554
Contributed by Tim Goodwin, Margi Butler, Russell Poulter
Comments DNA translated using the “alternative yeast nuclear” code (no. 12) results in 3 changes of Leu to Ser at 319, 396, 502 (Bulter).
References Butler 2005B

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