The Yeast Intein Database

The Yeast Intein Database is a comprehensive and curated database devoted to yeast inteins.


Inteins are self-catalytic protein splicing elements. The Yeast Intein Database and Registry, is a curated compilation of published and unpublished information about protein splicing. It presents general information as well as detailed data for each yeast intein, including tabulated comparisons and a comprehensive bibliography. An intein-specific BLAST server is now available to assist in identifying new inteins.

 

Intein Name Ctr VMA
Intein id YSTIN011
Intein Class Experimental
Prototype Allele Sce VMA
Organism Name Candida tropicalis (nucleus)
Exiein Name Vacuolar ATPase (H+-transporting ATP synthase), subunit A
Intein Class Experimental
Organism Description Yeast
Domain of Life Eucarya
Endonuclease Activity --
Endonuclease Motif DOD
Location in Extein G283
Inert Site Comment VMA-a
Intein Size 471
Intein N Terminal SNSDVIIYVG/C
Intein C Terminal HN/CGERGNEMAE
Accession No A46080 in NCBI/protein
Intein aa Sequence >gi|1076955|pir||A46080 Ctr VMA intein (including -1 and +1 extein residues) GCFTKGTQVMMADGADKSIESIEVGDKVMGKDGMPREVVGLPRGYDDMYKVRQLSSTRRNAKSEGLMDFT VSADHKLILKTKQDVKIATRKIGGNTYTGVTFYVLEKTKTGIELVKAKTKVFGHHIHGQNGAEEKAATFA AGIDSKEYIDWIIEARDYVQVDEIVKTSTTQMINPVHFESGKLGNWLHEHKQNKSLAPQLGYLLGTWAGI GNVKSSAFTMNSKDDVKLATRIMNYSSKLGMTCSSTESGELNVAENEEEFFNNLGAEKDEAGDFTFDEFT DAMDELTINVHGAAASKKNNLLWNALKSLGFRAKSTDIVKSIPQHIAVDDIVVRESLIAGLVDAAGNVET KSNGSIEAVVRTSFRHVARGLVKIAHSLGIESSINIKDTHIDAAGVRQEFACIVNLTGAPLAGVLSKCAL ARNQTPVVKFTRDPVLFNFDLIKSAKENYYGITLAEETDHQFLLSNMALVHNC
Block A CFTKGTQVMMADG 13
Block B LMDFTVSADHKLIL 78
Block C LLGTWAGIG 210
Block D VKSIPQHI 325
Block E LIAGLVDAAG 345
Block H TSFRHVARGLVKIAHSLGI 379
Block F YGITLA**EETDHQFL 462
Block G NMALVHNC 472
Contributed by Gary Dean
Comments The Ctr VMA extein gene has a typical Candida tropicalis intron and is the first gene reported to have both an intron and an intein (Gu 1993). Ctr VMA is a nuclear gene
References Gu 1993 Steuer 2004

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