The Yeast Intein Database

The Yeast Intein Database is a comprehensive and curated database devoted to yeast inteins.


Inteins are self-catalytic protein splicing elements. The Yeast Intein Database and Registry, is a curated compilation of published and unpublished information about protein splicing. It presents general information as well as detailed data for each yeast intein, including tabulated comparisons and a comprehensive bibliography. An intein-specific BLAST server is now available to assist in identifying new inteins.

 

Intein Name Cne-a RP8 (Fne-A PRP8)
Intein id YSTIN006
Intein Class Experimental
Prototype Allele Cne-AD PRP8
Organism Name Filobasidiella neoformans (Cryptococcus neoformans) Serotype A, PHLS_8104
Exiein Name PRP8, pre-mRNA splicing factor
Intein Class Experimental
Organism Description Yeast, human pathogen
Domain of Life Eucarya
Endonuclease Activity --
Endonuclease Motif None
Location in Extein A?
Inert Site Comment PRP8-a, same as A1578 in Sce PRP8
Intein Size 171
Intein N Terminal TWEGLFWEKA/C
Intein C Terminal HN/SGFEE-----
Accession No AAR00330in NCBI/protein
Intein aa Sequence >gi|37695591|gb|AAR00330.1|[37695591] Cne-A PRP8 (Fne-A PRP8) intein (including -1 and +1 extein residues) ACLQNGTRLLRADGSEVLVEDVQEGDQLLGPDGTSRTASKIVRGEERLYRIKTHEGLEDLVCTHNHILSM YKERFGREGAHSPSAGTSLTESHERVDVTVDDFVRLPQQEQQKYKLFRSTDFVRREQPSASKLATLLHIN SIELEEEPTKWSGFVVDKDSLYLRYDYLVLHNS
Block A CLQNGTRLLRADG 13
Block B LEDLVCTHNHILSM 69
Block C None
Block D None
Block E None
Block H None
Block F PTKWSGFVVDKDSLYL 162
Block G DYLVLHNS 172
Contributed by Margi Butler
Comments The organism is also called Cryptococcus neoformans var grubii. The Fne PRP8 gene has not yet been fully described. This gene also contains an intron. Same aa seq for Cne strains Cn 3511 & JEC 21, with 1 silent nt change (Butler 2001).Same intein sequence in strain H99 and IUM93-3231
References Butler 2001 Butler 2005 Loftus 2005

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