The Yeast Intein Database

The Yeast Intein Database is a comprehensive and curated database devoted to yeast inteins.


Inteins are self-catalytic protein splicing elements. The Yeast Intein Database and Registry, is a curated compilation of published and unpublished information about protein splicing. It presents general information as well as detailed data for each yeast intein, including tabulated comparisons and a comprehensive bibliography. An intein-specific BLAST server is now available to assist in identifying new inteins.

 

Intein Name Cga PRP8
Intein id YSTIN004
Intein Class Theoretical
Prototype Allele Cne-AD PRP8
Organism Name Cryptococcus gattii (aka Cryptococcus bacillisporus)
Exiein Name PRP8, pre-mRNA splicing factor
Intein Class Theoretical
Organism Description Yeast, human pathogen
Domain of Life Eucarya
Endonuclease Activity --
Endonuclease Motif None
Location in Extein A?
Inert Site Comment PRP8-a, same as A1578 in Sce PRP8
Intein Size 170
Intein N Terminal TWEGLFWEKA/C
Intein C Terminal HN/SGFEESMKNK
Accession No AAR00331.1 in NCBI/protein
Intein aa Sequence >gi|37695593|gb|AAR00331.1|CbaPRP8_WM02.98| Cga PRP8 intein (including -1 and +1 extein residues) ACLQNGTRLLRADGSEILVEDVQEGDQLLGPDGTSRTASKIVRGEERLYRIKADELEDLVCTHNHILSL YKKRSGSEQDPSPSTDLSSTDSYERVDVTVDDFVRLPQQEQQKYRLFRSTGFKRADQPSTSSLATLLHI MSIQLEEKPTKWSGFVVVKDSLYLRHDYLVLHNS
Block A CLQNGTRLLRADG 13
Block B LEDLVCTHNHILSL 68
Block C None
Block D None
Block E None
Block H None
Block F PTKWSGFVVDKDSLYL 161
Block G DYLVLHNS 171
Contributed by Margi Butler
Comments The organism is also called Cryptococcus neoformans var gattii and Cryptococcus bacillisporus. This gene also contains an intron. The PRP8 inteins from numerous Cga strains form 4 clades based on DNA sequence
References Butler 2005

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