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Most
enzymes make slightly staggered incisions, resulting
in "sticky ends", out of which one strand
protrudes.Incisions where the 3' end protrudes are known
as 3'overhangs or 3'sticky ends. An example would be
the enzyme Sac I:
Sac
I searches for the sequence GAGCTC on each strand
(once again, GAGCTC reads the same off of both strands
because the sequence is palindromic). The enzyme breaks
the phosphodiester bonds between the fifth and sixth
nucleotides in the recognition sequence.
5'-GAGCTC-3'
Sac I 5'-GAGCT -3' + 5'- C-3'
3'-CTCGAG-5' ----> 3'-C -5' 3'- TCGAG-5'
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