The Restriction EnzyBase Project
Bioinformatics Research Lab.
IBI Biosolutions Pvt. Ltd., India
IBI Biosolutions Pvt. Ltd.
www.ibibiosolutions.com
I-SceI
Restriction Enzyme Name
I-SceI
Methyltranferases
Recognition Sequence
AGTTACGCTAGGGATAACAGGGTAATATAG (-13/-17)
Type
Homing
Subtype
-
Prototype
I-SceI
Organism
Saccharomyces cerevisiae
Temperature
30 °
Molecular weight
27682
Blunt End
3' Sticky End
5' Sticky End
Length of Overhang
Outside Cutter
Isochizomers
-
Synonyms
omega
Suppliers
Fermentas International Inc., New England Biolabs
Cloned/sequenced
cloned and sequenced
Specificity Subunit
Nicking Enzymes
Methyl directed
Homing enzymes
yes
Star Activity
Single stranded cleavages
Orphan methyltransferases
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