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p53
Structures
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Detail Information of mai58g03.y1 EST:
| dbEST Id | 16973715 |
| Organ | testis |
| sequencing | Primer name ambiguous |
| Tissue type | spermatocytes, pooled from multiple mice |
| EST name | mai58g03.y1 |
| GenBank Acc | CB272467 |
| GenBank gi | 28462790 |
| Clone Id | IMAGE:6445733 (5') |
| DNA type | cDNA |
| PolyA Tail | Unknown |
| Sequence | GCACGAGGAGAAGAGAACGTTGAAGCAGCCCTTCGCTAGCGGCCGCGGTGACAGCCCGGC TCCGGCGCGATGAACCGCGGTGGCAGCAGCGCGTCGGCCTCGGCCAACTACCTGCTCTGC ACCAACTGCCGGAAAGTGCTGCGGAAGGACAAAAGAATCCGAGTGTCTCAACCCCTGACA AGAGGACCAAGTGCCTTTATTCCAGAGAAGGAAGTTGTCCAAGCAAATACGGCGGATGAA CGAACAAACTTTCTTGTGGAAGAGTACTCTACATCCGGTCGCCTGGACAACATCACACAG GTGATGAGCTTGCACACTCAGTACCTGGAATCTTTCTTGCGGAGCCAGTTCTACATGCTG CGTATGGACGGCCCCCTTCCCCTCCCAGACAGGCACTACATTGCCATCATGGCTGCAGCC AGAC |
| vector | pBluescript SK+ (Stratagene) |
| Rsite1 | XhoI |
| Rsite2 | EcoRI |
| Description | cDNA oligo dT-primed [5'-(GA)10-ACTAGTCTCGAGTTTTTTTTTTTTT-3' ] and directionally cloned using 5' linkers 5'-AATTCGGCACGAG-3' and 5'-CTCGTGCCG-3'. Size selection of >400bp material gives average insert size ranging from 1-2 kb. Library was mass excised (from lambda-UniZAP-XR) and resulting single-stranded phagemids were prepped and tranformed into DH10B. Library contains 98% recombinants. References: J. Androl. 20:635-639 and Gene 25:263-269. Library constructed and donated by J. McCarrey, Ph.D. (Southwest Foundation for Biomedical Research, Dept. of Genetics); excision done by E.M. Eddy, Ph.D. (National Institutes of Health, National Institute of Environmental Health Sciences). Original lambda-based library is available through ATCC, catalog #63422. |
| Putative ID | Assigned by submitter TR:Q9Y6P6 Q9Y6P6 P53 REGULATED PA26-T2 NUCLEAR PROTEIN. |
| Lib Name | McCarrey Eddy spermatocytes |
| Organism | Mus musculus |