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p53
Structures
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Detail Information of PDUts2042F05 EST:
| dbEST Id | 26676255 |
| Organ | testis |
| sequencing | T7 promoter primer |
| Tissue type | |
| EST name | PDUts2042F05 |
| GenBank Acc | CX060122 |
| GenBank gi | 84136264 |
| Clone Id | PDUts2042F05 (5') |
| DNA type | cDNA |
| PolyA Tail | no |
| Sequence | GACACGAGCTGTGGCGGAGCTTGTTGGAGTCCCTGCTGAACTCACTCCGGACTGGAGCGT ACTATGCTGTCTCCCGAGCCGGAGCGCGTGCTGCAGTACCTGCTGCAGGTGGAGGAGCTC GCCGAAGAGGTTCTGGAGGACGATCTGGCGATTGTGCACCTGAACGCCGACCTGAATCCC AACCGGGAGGGCCTGAGGAGCCCTGCTGAAAGATCTCAGCCTCTCTGACGATGTGATGGA TTGCTTCATGAACATGTTTATCAAGATGCCTCTCCCAAAGACTAACCAAATGATTGAGAA AGATCTTGACCATCTGAATGACCATCTAGATAGACTCCTGAAACGACTTCGGGTGCAGGT CAACCGCCTCTTT |
| vector | pSPORT1 |
| Rsite1 | NotI |
| Rsite2 | SalI |
| Description | Material with normal function was tested and heat stress experiments of animals were conducted by Divisions of Biotechnology and Animal Resources, Animal Technology Institute Taiwan (ATIT). First strand cDNA was primed with a NotI-oligo(dT) primer and 2nd strand cDNA was synthesized by using nick translational replacement of the mRNA. Double-strand cDNA was added with SalI adapter, then digested with NotI and cloned into the NotI and SalI sites of pSPORT1 vector. |
| Putative ID | Assigned by submitter homologue to ref|NM_030815.2| Homo sapiens p53 and DNA damage regulated 1 (PDRG1), mRNA |
| Lib Name | Porcine testis cDNA library II |
| Organism | Sus scrofa |