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p53
Structures
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Detail Information of DAY35S_07_H11.x1 EST:
| dbEST Id | 32052423 |
| Organ | prostate |
| sequencing | |
| Tissue type | Equal amounts of pooled ventral prostate, dorsolateral prostate, and coagulating gland (anterior prostate) from five 35 day old male mice, subtracted |
| EST name | DAY35S_07_H11.x1 |
| GenBank Acc | DV049931 |
| GenBank gi | 76377214 |
| Clone Id | DAY35S_07_H11 |
| DNA type | cDNA |
| PolyA Tail | Unknown |
| Sequence | TCCGCCCTTTATTGGGCGGGATAACGTCTTAGGTGCACAAGGACCCTACCAGAGCTCCTG GGTGGCTTTGTGTGAGGGGACTGAACGCAGACAAACCACAGCCTGCTTCTAATACCTTCT TTCCCTACCACCTAGTTCCAAATGGAACCAACAAGTTGAGTGCATCTCTGTTGGGTGTTT TGTGTTGAGACTGGCTGAAGTGAAGAATCTTTGACTGACCATGTTGTGATGTGTCGACAG ACTCAAGGACACAACCACCTCGACCTGGTCATGTGGCATGCCTGTGTATGTGTGTAACAG GATTCTGAATGTTAGGTTGTAATGCTATTCCTGTATGGGAGAAAAAATAATATAAACAAA TAAAAATCTATTTAAAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATTTTTTTGTGCCCC TTTTCCCCCTTTTTTTTTTTTTTTGGAAAATTTTTTTTTTGGGGCCCCATTTTGGTCCTT TTTGGCCAATTCCCCTTTTGGGGGGGGCAAAAAACCGGCGGTTCTTTTCTTTTTTTCTTC CTGTAATCTTCTTAATTTTTTGTTCTTCTCCCAATTGACGTGCCTACTACGTGTACCCGT CTACCTCATATCTTTATGCGGAAAAACAGTGGGTATTTGTCAATCCAAGTTGGGTCGCAC TGTTTTTAACCGTATTCAGTATACCTTCTTGACGGGGCTTGTTTTATCGCTACACACGCT TGATCTGTTTTATGACTATCAGCAGTAACATTGCACACTAGTGTTTTTAATCTTCATGTG CATTCTACTAATTTCACCTGTGTGTGTTTTATTATCTTGACCCTTCATCTTTTTTCCTGC ATTACTTTTATATTTTTGCCCCCATTTTTTGTACCCCCGTTCATGACTTATTTTCAGCCC TCGTTTTTTTATTCCCATCGCGTTCCCCTGTATTGTGTACCG |
| vector | pTriplEx2 |
| Rsite1 | SfiI |
| Rsite2 | SfiI |
| Description | Library was constructed using Clontechs SMART cDNA library construction kit. 0.5ug of total RNA from pooled 35-day dorsolateral prostate, 0.5ug of total RNA from pooled 35-day ventral prostate, and 0.5ug of total RNA from pooled 35-day coagulating gland (anterior prostate ) was combined and used in 1st strand cDNA synthesis. 2nd strand cDNA was generated by 21 cycles of PCR. One prominent band from the double stranded cDNA was removed from the gel and the remaining cDNA was gel isolated. This subtracted cDNA was digested with SfiI and ligated into the lambdaTriplEx2 phagemid. Phagemids were converted to pTriplEx2 plasmids in the BM25.8 e-coli strain. Created from pooled prostate from five 35 day old male C57BL6 mice by Colin Pritchard. |
| Putative ID | Assigned by submitter Tumor protein p53 inducible nuclear protein 2 |
| Lib Name | FH DAY35S |
| Organism | Mus musculus |