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p53
Structures
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Detail Information of BB560972 EST:
| dbEST Id | 5637169 |
| Organ | |
| sequencing | |
| Tissue type | olfactory brain |
| EST name | BB560972 |
| GenBank Acc | BB560972 |
| GenBank gi | 9647338 |
| Clone Id | E530119A01 (3') |
| DNA type | cDNA |
| PolyA Tail | Unknown |
| Sequence | CCGTTCACGAGAAGATGGCCATAATGAACTCATACATTATTTATTCACTCTCGGATTACG ACCCCCACCAAGACGATCAGCTGCTCATGAAAGAAGGAGATTGCATGACCTTCATCCGCA GAGAAGATGAAGAGGAGATCGAGTGGTGGTGGGCCCCCCTTAACGATAAGGAAGGATATG TTCCACGCAACTTGCTAGGACTATACCCAAGAATTAAACCTAGACAAAGGAGCTTGCCCT GAAACTTGAGTTAATGAAGAATTAATGAGCTAGAAGAAATACTATGATTATCC |
| vector | |
| Rsite1 | SalI |
| Rsite2 | BamHI |
| Description | cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAGCGGCCGCAACTCGAGTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cleaved with BamHI and XhoI. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I. |
| Putative ID | Assigned by submitter U58334 Human Bcl2, p53 binding protein Bbp/53BP2 (BBP/53BP2) mRNA |
| Lib Name | RIKEN full-length enriched, 10 days neonate olfactory brain |
| Organism | Mus musculus |