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p53 Structures |
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Rat p53
No monoclonal have been developped toward rat p53.
Most of the monoclonal antibodies that binds to murine p53 binds also to rat p53.
There are a few exceptions with monoclonal antibodies which binds to rat p53 but does not recognize murine p53 (table 2)
Table 1a : Rat and mouse p53 monoclonal antibodies
(mouse p53 used for immunization)
Name |
Epitope Location |
Antigen used for immunization |
Isotype |
Other species recognition |
WB |
IP |
Reference |
Comercial availability |
PAb242 |
18-27 |
Mouse p53 |
IgG1 |
No |
Yes |
Yes |
6, 16 |
|
PAb248 |
43-52 |
Mouse p53 |
IgG2b |
No |
Yes |
Yes |
4, 6, 16 |
|
200.47 |
78-87 |
Mouse p53 |
IgG2a |
weak human (1) |
Yes |
Yes |
20, 16 |
No |
PAb240 |
213-217 |
Mouse p53 |
IgG1 |
Yes |
Yes |
Yes |
6, 7, 8 |
Yes |
PAb122 |
371-380 |
Mouse p53 |
IgG2b |
Yes |
Yes |
Yes |
1,4,15 |
Yes |
PAb421 |
371-380 |
Mouse p53 |
IgG2a |
Yes |
Yes |
Yes |
2,4,15 |
Yes |
PAb246 |
?(2) |
Mouse p53 |
IgG1 |
No |
No |
Yes |
6, 7, 16 |
Yes |
Only mAbs that have been experimentally tested either by ELISA, immunoprecipitation or western blot are listed on these tables.
From the epitope sequence, it is quite possible that other mAbs react with this species.
( 1) Recognition based on peptide binding. Cross reaction of 200.27 to human p53 has been the subject of debate (see references )
(2) PAb246, like PAb1620, binds a conformational epitopes wich disapears upon denaturation or in some mutant p53
Table 1b : Rat and mouse p53 monoclonal antibodies
(human p53 used for immunization)
Name |
Epitope Location |
Antigen used for immunization |
Isotype |
Other species recognition |
WB |
IP |
Reference |
Comercial availability |
B17 |
16-25 |
Human p53 |
IgG2a |
Yes |
Yes |
Yes |
11, 12 |
No |
X77 |
16-25 |
Xenopus p53 |
IgG1 |
Yes |
Yes |
Yes |
18 |
No |
X73 |
16-25 |
Xenopus p53 |
IgG1 |
Yes |
Yes |
Yes |
18 |
No |
X18 |
16-25 |
Xenopus p53 |
IgG1 |
Yes |
Yes |
Yes |
18 |
No |
HP64 |
171-185 |
Human p53 |
IgG1 |
Yes |
Yes |
Yes |
11, 12 |
No |
HO13.1 |
211-220 |
Human p53 |
IgG2b |
Yes |
Yes |
Yes |
13 |
No |
HO8.1 |
306-361 |
Human p53 |
IgG1 |
Yes |
Yes |
Yes |
13 |
Yes |
HR231 |
371-380 |
Human p53 |
IgG2b |
Yes |
Yes |
Yes |
11, 12 |
No |
Only mAbs that have been experimentally tested either by ELISA, immunoprecipitation or western blot are listed on these tables.
From the epitope sequence, it is quite possible that other mAbs react with this species.
Table 2 : Mabs that distinguish rat from mouse p53
Name |
Epitope Location |
Antigen used for immunization |
Isotype |
Other species recognition |
WB |
IP |
Reference |
Comercial availability |
C36 |
16-30 |
Human p53 |
IgG1 |
Yes |
Yes |
Yes |
11, 12 |
No |
H461 |
16-30 |
Human p53 |
IgG1 |
Yes |
Yes |
Yes |
11, 12 |
No |
X87 |
16-30 |
Xenopus p53 |
IgG1 |
Yes |
Yes |
Yes |
19 |
No |
X44 |
16-30 |
Xenopus p53 |
IgG1 |
Yes |
Yes |
Yes |
19 |
No |
X61 |
16-30 |
Xenopus p53 |
IgG1 |
Yes |
Yes |
Yes |
19 |
No |
X91 |
16-30 |
Xenopus p53 |
IgG1 |
Yes |
Yes |
Yes |
19 |
No |
Only mAbs that have been experimentally tested either by ELISA, immunoprecipitation or western blot are listed on these tables.
From the epitope sequence, it is quite possible that other mAbs react with this species.