HOMO SAPIENS WITH MUS MUSCULUS

MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGP
MEE QSD S+E PLSQETFS LWKLLP  ++L P P   MDDL+L P D+E++F    GP
MEESQSDISLELPLSQETFSGLWKLLPPEDIL-PSP-HCMDDLLL-PQDVEEFFE---GP

DEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAK
 EA R+  A     P P P    APAPA  WPLSS VPSQKTYQG+YGF LGFL SGTAK
SEALRVSGAPAAQDPVTETPGPVAPAPATPWPLSSFVPSQKTYQGNYGFHLGFLQSGTAK

SVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHE
SV CTYSP LNK+FCQLAKTCPVQLWV +TPP G+RVRAMAIYK+SQHMTEVVRRCPHHE
SVMCTYSPPLNKLFCQLAKTCPVQLWVSATPPAGSRVRAMAIYKKSQHMTEVVRRCPHHE

RCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNS
RCSD DGLAPPQHLIRVEGNL EYL+DR TFRHSVVVPYEPPE  GS+ TTIHY YMCNS
RCSDGDGLAPPQHLIRVEGNLYPEYLEDRQTFRHSVVVPYEPPEAGSEYTTIHYKYMCNS

SCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELP
SCMGGMNRRPILTIITLEDSSGNLLGR+SFEVRVCACPGRDRRTEEEN RKK     ELP
SCMGGMNRRPILTIITLEDSSGNLLGRDSFEVRVCACPGRDRRTEEENFRKKEVLCPELP

PGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPG
PGS KRALP TS+SP KKKPLDGEYFTL+IRGR+RFEMFRELNEALELKDA A   +E G
PGSAKRALPTCTSASPPQKKKPLDGEYFTLKIRGRKRFEMFRELNEALELKDAHATEESG

GSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD
 SRAHSS+LK+KKGQSTSRHKK M K  GPDSD
DSRAHSSYLKTKKGQSTSRHKKTMVKKVGPDSD

HOMO SAPIENS WITH RATTUS NORVEGICUS

MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPS---QAMDDLMLSPDDIEQWFTED
ME+ QSD S+E PLSQETFS LWKLLP +++L    +    +M+DL L P D+ +
MEDSQSDMSIELPLSQETFSCLWKLLPPDDILPTTATGSPNSMEDLFL-PQDVAELLE--

PGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSG
 GP+EA ++   A       A    A + P   WPLSSSVPSQKTYQG+YGF LGFL SG
-GPEEALQVSAPAAQEPGTEAPAPVAPASATP-WPLSSSVPSQKTYQGNYGFHLGFLQSG

TAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCP
TAKSV CTYS +LNK+FCQLAKTCPVQLWV STPPPGTRVRAMAIYK+SQHMTEVVRR P
TAKSVMCTYSISLNKLFCQLAKTCPVQLWVTSTPPPGTRVRAMAIYKKSQHMTEVVRRWP

HHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYM
HHERCSD DGLAPPQHLIRVEGN   EYLDDR TFRHSVVVPYEPPEVGSD TTIHY YM
HHERCSDGDGLAPPQHLIRVEGNPYAEYLDDRQTFRHSVVVPYEPPEVGSDYTTIHYKYM

CNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHH
CNSSCMGGMNRRPILTIITLEDSSGNLLGR+SFEVRVCACPGRDRRTEEEN RKK E
CNSSCMGGMNRRPILTIITLEDSSGNLLGRDSFEVRVCACPGRDRRTEEENFRKKEEHCP

ELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGK
ELPPGS KRALP +TSSSPQ KKKPLDGEYFTL+IRGRERFEMFRELNEALELKDA+A +
ELPPGSAKRALPTSTSSSPQQKKKPLDGEYFTLKIRGRERFEMFRELNEALELKDARAAE

EPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD
E G SRAHSS+ K+KKGQSTSRHKK M K  GPDSD
ESGDSRAHSSYPKTKKGQSTSRHKKPMIKKVGPDSD

HOMO SAPIENS WITH CANIS FAMILIARIS

MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGP
MEE QS+ +++PPLSQETFS+LW LLPENNVLS     A+D+L+L P+ +  W  ED
MEESQSELNIDPPLSQETFSELWNLLPENNVLSSELCPAVDELLL-PESVVNWLDEDSDD

DEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAK
                PA   P AP PAPSWPLSSSVPS KTY G+YGFRLGFLHSGTAK
------------APRMPATSAPTAPGPAPSWPLSSSVPSPKTYPGTYGFRLGFLHSGTAK

SVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHE
SVT TYSP LNK+FCQLAKTCPVQLWV S PPP T VRAMAIYK+S+ +TEVVRRCPHHE
SVTWTYSPLLNKLFCQLAKTCPVQLWVSSPPPPNTCVRAMAIYKKSEFVTEVVRRCPHHE

RCSDS-DGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCN
RCSDS DGLAPPQHLIRVEGNLR +YLDDRNTFRHSVVVPYEPPEVGSD TTIHYNYMCN
RCSDSSDGLAPPQHLIRVEGNLRAKYLDDRNTFRHSVVVPYEPPEVGSDYTTIHYNYMCN

SSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHEL
SSCMGGMNRRPILTIITLEDSSGN+LGRNSFEVRVCACPGRDRRTEEEN  KKGEP  E
SSCMGGMNRRPILTIITLEDSSGNVLGRNSFEVRVCACPGRDRRTEEENFHKKGEPCPEP

PPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEP
PPGSTKRALP +TSSSP  KKKPLDGEYFTLQIRGRER+EMFR LNEALELKDAQ+GKEP
PPGSTKRALPPSTSSSPPQKKKPLDGEYFTLQIRGRERYEMFRNLNEALELKDAQSGKEP

GGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD
GGSRAHSSHLK+KKGQSTSRHKKLMFK EG DSD
GGSRAHSSHLKAKKGQSTSRHKKLMFKREGLDSD

HOMO SAPIENS WITH SUS SCROFA

MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSP-LPSQAMDDLMLSPDDIEQWFTEDPG
MEE QS+  VEPPLSQETFSDLWKLLPENN+LS  L   A++DL+LSP +   W  E+P
MEESQSELGVEPPLSQETFSDLWKLLPENNLLSSELSLAAVNDLLLSP--VTNWLDENPD

PDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTA
       P AA   APA  AP         SWPLSS VPSQKTY GSY FRLGFLHSGTA
DASRVPAPPAATAPAPAAPAPAT-------SWPLSSFVPSQKTYPGSYDFRLGFLHSGTA

KSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHH
KSVTCTYSPALNK+FCQLAKTCPVQLWV S PPPGTRVRAMAIYK+S++MTEVVRRCPHH
KSVTCTYSPALNKLFCQLAKTCPVQLWVSSPPPPGTRVRAMAIYKKSEYMTEVVRRCPHH

ERCSD-SDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMC
ER SD SDGLAPPQHLIRVEGNLR EYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYN+MC
ERSSDYSDGLAPPQHLIRVEGNLRAEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNFMC

NSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHE
NSSCMGGMNRRPILTIITLED+SGNLLGRNSFEVRVCACPGRDRRTEEEN  KKG+   E
NSSCMGGMNRRPILTIITLEDASGNLLGRNSFEVRVCACPGRDRRTEEENFLKKGQSCPE

LPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKE
 PPGSTKRALP +TSSSP  KKKPLDGEYFTLQIRGRERFEMFRELN+ALELKDAQ  +E
PPPGSTKRALPTSTSSSPVQKKKPLDGEYFTLQIRGRERFEMFRELNDALELKDAQTARE

PGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD
G  +RAHSSHLKSKKGQS SRHKK MFK EGPDSD
SGENRAHSSHLKSKKGQSPSRHKKPMFKREGPDSD

HOMO SAPIENS WITH BOS TAURUS

MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGP
MEE Q++ +VEPPLSQETFSDLW LLPENN+LS   S  +DDL L   D+  W  E P
MEESQAELNVEPPLSQETFSDLWNLLPENNLLSSELSAPVDDL-LPYTDVATWLDECPNE

DEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAK
           P   APAAP PA PAPA SWPLSS VPSQKTY G+YGFRLGFL SGTAK
-------APQMPEPSAPAAPPPATPAPATSWPLSSFVPSQKTYPGNYGFRLGFLQSGTAK

SVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHE
SVTCTYSP+LNK+FCQLAKTCPVQLWVDS PPPGTRVRAMAIYK+ +HMTEVVRRCPHHE
SVTCTYSPSLNKLFCQLAKTCPVQLWVDSPPPPGTRVRAMAIYKKLEHMTEVVRRCPHHE

RCSD-SDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCN
R SD SDGLAPPQHLIRVEGNLR EYLDDRNTFRHSVVVPYE PE+ S+CTTIHYN+MCN
RSSDYSDGLAPPQHLIRVEGNLRAEYLDDRNTFRHSVVVPYESPEIDSECTTIHYNFMCN

SSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHEL
SSCMGGMNRRPILTIITLEDS GNLLGRNSFEVRVCACPGRDRRTEEENLRKKG+   E
SSCMGGMNRRPILTIITLEDSCGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGQSCPEP

PPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEP
PP STKRALP NTSSSPQPKKKPLDGEYFTLQIRG +R+EMFRELN+ALELKDA  G+EP
PPRSTKRALPTNTSSSPQPKKKPLDGEYFTLQIRGFKRYEMFRELNDALELKDALDGREP

GGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD
G SRAHSSHLKSKK  S S HKK M K EGPDSD
GESRAHSSHLKSKKRPSPSCHKKPMLKREGPDSD

HOMO SAPIENS WITH PAN TROGLODYTES

MFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQ MFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQ MFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQ

HLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPIL HLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPIL HLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPIL

TIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNT TIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNT TIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNT

SSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSK SSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSK SSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSK

KGQSTSRHKKLMFKTEGPDSD
KGQSTSRHKKLMFKTEGPDSD
KGQSTSRHKKLMFKTEGPDSD

N-TERMINAL TRANSCRIPTION ACTIVATION DOMAIN

The binding of this protein by regulatory proteins regulates p53 transcription activation. his entry is comprised of a single amphipathic alpha helix and contains a highly conserved motif. It present on the 5 to 29 position in the protien. In the analysis we found that there is some mutations in diffrent organisms but these mutations did not affect the function of this protein.In this alignment this domain shows in blue colour.

DNA BINDING DOMAIN

This domain is found in p53 transcription factor, where it is responsible for DNA-binding. These transcription factors play diverse roles in the regulation of cellular functions: the p53 tumour suppressor upregulates the expression of genes involved in cell cycle arrest and apoptosis he DNA-binding domain acts to clamp, or in the case of TonEBP, encircle the DNA target in order to stabilize the protein-DNA complex. Protein interactions may also serve to stabilize the protein-DNA complex, for example in the STAT-1 dimer the SH2 (Src homology 2) domain in each monomer is coupled to the DNA-binding domain to increase stability The DNA-binding domain consists of a beta-sandwich formed of 9 strands in 2 sheets with a Greek-key topology. This structure is found in many transcription factors, often within the DNA-binding domain.we analyse that there are very less mutation in these organism so that the function of this protien did not changed in these organisms.In this alignment this domain shows in pink colour.

Crystal Structure of Core Domain

PROLINE RICH DOMAIN

This Domain is responsible for the apoptotic activity of the protein. These regions are often mosiacs of a small number of amino acids. These regions have been shown to be functioanlly important in some proteins. this domain present at the 60 to 89 position in the protein.In this alignment this domain shows in green colour.

TETRAMERISATION DOMAIN

The p53 protein is a tetrameric transcription factor that plays a central role in the prevention of neoplastic transformation. Oligomerization appears to be essential for the tumour suppressing activity of p53. p53 can be divided into different functional domains: an N-terminal transactivation domain, a proline-rich domain, a DNA-binding domain (), a tetramerisation domain and a C-terminal regulatory region. The tetramerisation domain of human p53 extends from residues 325 to 356, and has a 4-helical bundle fold. The tetramerisation domain is essential for DNA binding, protein-protein interactions, post-translational modifications, and p53 degradation.this domain is same in all seven organism so the function of protein did not changed. In this alignment this domain shows in red colour.