p53 Database
p53 Structures

Mouse p53

Table 1 : monoclonal antibodies developped toward murine p53

Name

Epitope Location

Antigen used

for immunization

Isotype

Other species

recognition

WB

IP

Reference

Comercial

availability

PAb242

18-27

Mouse p53

IgG1

No

Yes

Yes

6, 17

No

RA32C2

23-32 & 23-47

Mouse p53

Rat IgM

weak human p53 ?

Yes

Yes

20, 17

No

PAb248

43-52

Mouse p53

IgG2b

No

Yes

Yes

4, 6, 17

No

200.47

78-87

Mouse p53

IgG2a

weak human (1)

Yes

Yes

21, 17

No

PAb240

213-217

Mouse p53

IgG1

Yes

Yes

Yes

6, 7, 8

Yes

Pab241

288-297

Mouse p53

IgG2a

weak human (1)

Yes

Yes

17

No

Pab243

288-297

Mouse p53

IgG2a

weak human (1)

Yes

Yes

17

No

PAb122

371-380

Mouse p53

IgG2b

Yes

Yes

Yes

1,4,15

Yes

PAb421

371-380

Mouse p53

IgG2a

Yes

Yes

Yes

2,4,15

Yes

PAb246

?(2)

Mouse p53

IgG1

No

No

Yes

6, 7, 17

Yes

Only mAbs that have been experimentally tested either by ELISA, immunoprecipitation or western blot are listed on these tables.
From the epitope sequence, it is quite possible that other mAbs react with this species.

 ( 1) Recognition based on peptide binding. Cross reaction of 200.27 to human p53 has been the subject of debate (see references )
(2) PAb246, like PAb1620, binds a conformational epitopes wich disapears upon denaturation or in some mutant p53

Table 2 : monoclonal antibodies developped toward other p53 but that cross react with murine p53

Name

Epitope Location

Antigen used

for immunization

Isotype

Other species

recognition

WB

IP

Reference

Comercial

availability

B17

16-25

Human p53

IgG2a

Yes

Yes

Yes

11, 12

No

X77

16-25

Xenopus p53

IgG1

Yes

Yes

Yes

19

No

X73

16-25

Xenopus p53

IgG1

Yes

Yes

Yes

19

No

X18

16-25

Xenopus p53

IgG1

Yes

Yes

Yes

19

No

HP64

171-185

Human p53

IgG1

Yes

Yes

Yes

11, 12

No

HO13.1

211-220

Human p53

IgG2b

Yes

Yes

Yes

13

No

HO8.1

306-361

Human p53

IgG1

Yes

Yes

Yes

13

Yes

HR231

371-380

Human p53

IgG2b

Yes

Yes

Yes

11, 12

No

Only mAbs that have been experimentally tested either by ELISA, immunoprecipitation or western blot are listed on these tables.
From the epitope sequence, it is quite possible that other mAbs react with this species.

 

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