| p53 Database |
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p53 Structures |
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Mammals |
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© 2007 IBI Biosolutions Pvt. Ltd. |
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Mouse p53
Table 1 : monoclonal antibodies developped toward murine p53
Name |
Epitope Location |
Antigen used
for immunization |
Isotype |
Other species
recognition |
WB |
IP |
Reference |
Comercial
availability |
PAb242 |
18-27 |
Mouse p53 |
IgG1 |
No |
Yes |
Yes |
6, 17 |
No |
RA32C2 |
23-32 & 23-47 |
Mouse p53 |
Rat IgM |
weak human p53 ? |
Yes |
Yes |
20, 17 |
No |
PAb248 |
43-52 |
Mouse p53 |
IgG2b |
No |
Yes |
Yes |
4, 6, 17 |
No |
200.47 |
78-87 |
Mouse p53 |
IgG2a |
weak human (1) |
Yes |
Yes |
21, 17 |
No |
PAb240 |
213-217 |
Mouse p53 |
IgG1 |
Yes |
Yes |
Yes |
6, 7, 8 |
Yes |
Pab241 |
288-297 |
Mouse p53 |
IgG2a |
weak human (1) |
Yes |
Yes |
17 |
No |
Pab243 |
288-297 |
Mouse p53 |
IgG2a |
weak human (1) |
Yes |
Yes |
17 |
No |
PAb122 |
371-380 |
Mouse p53 |
IgG2b |
Yes |
Yes |
Yes |
1,4,15 |
Yes |
PAb421 |
371-380 |
Mouse p53 |
IgG2a |
Yes |
Yes |
Yes |
2,4,15 |
Yes |
PAb246 |
?(2) |
Mouse p53 |
IgG1 |
No |
No |
Yes |
6, 7, 17 |
Yes |
Only mAbs that have been experimentally tested either by ELISA, immunoprecipitation or western blot are listed on these tables.
From the epitope sequence, it is quite possible that other mAbs react with this species.
( 1) Recognition based on peptide binding. Cross reaction of 200.27 to human p53 has been the subject of debate (see references )
(2) PAb246, like PAb1620, binds a conformational epitopes wich disapears upon denaturation or in some mutant p53
Table 2 : monoclonal antibodies developped toward other p53 but that cross react with murine p53
Name |
Epitope Location |
Antigen used
for immunization |
Isotype |
Other species
recognition |
WB |
IP |
Reference |
Comercial
availability |
B17 |
16-25 |
Human p53 |
IgG2a |
Yes |
Yes |
Yes |
11, 12 |
No |
X77 |
16-25 |
Xenopus p53 |
IgG1 |
Yes |
Yes |
Yes |
19 |
No |
X73 |
16-25 |
Xenopus p53 |
IgG1 |
Yes |
Yes |
Yes |
19 |
No |
X18 |
16-25 |
Xenopus p53 |
IgG1 |
Yes |
Yes |
Yes |
19 |
No |
HP64 |
171-185 |
Human p53 |
IgG1 |
Yes |
Yes |
Yes |
11, 12 |
No |
HO13.1 |
211-220 |
Human p53 |
IgG2b |
Yes |
Yes |
Yes |
13 |
No |
HO8.1 |
306-361 |
Human p53 |
IgG1 |
Yes |
Yes |
Yes |
13 |
Yes |
HR231 |
371-380 |
Human p53 |
IgG2b |
Yes |
Yes |
Yes |
11, 12 |
No |
Only mAbs that have been experimentally tested either by ELISA, immunoprecipitation or western blot are listed on these tables.
From the epitope sequence, it is quite possible that other mAbs react with this species.
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