| p53 Database |
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p53 Structures |
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Mammals |
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© 2007 IBI Biosolutions Pvt. Ltd. |
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Sus
scrofa is the wild ancestor of the domestic pig. It
is native in woodlands across much of Central Europe , the Mediterranean
Region (including North Africa 's Atlas Mountains ) and much of
Asia as far south as Indonesia , and has been widely introduced
elsewhere. It is in the same Suidae biological family as the Warthog
and Bushpig of Africa, the Pygmy Hog of northern India , Babirusa
of Indonesia and others.
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Description
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Feral pigs in Texas are descended
from introductions of European wild hogs for sporting purposes,
and from escaped domestic swine that have established feral populations.
European wild hogs have several distinguishing characteristics
that set them apart from domestic or feral hogs. Among these are
brown to blackish brown color, with grizzled guard hairs, a mane
of hair (8-16 cm long) running dorsally from the neck to the rump,
a straight heavily tufted tail, and ears covered with hair. Characteristics
of feral hogs are varied, depending upon the breed of the ancestral
stock. European wild hogs and feral hogs interbreed readily, with
traits of European wild hogs apparently being dominant.
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| Habits
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Good feral hog habitat
in timbered areas consists of diverse forests with some openings.
The presence of a good litter layer to support soil invertebrates
and/or the presence of ground vegetation affording green forage,
roots, and tubers is desirable. Hogs are also fond of marsh and
grass-sedge flats in coastal areas, particularly if wild grapes
are common. During hot summer months, "wallows," or
depressions dug in the mud by feral hogs, are much in evidence
near marshes or standing water, such as along roadside ditches.
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On the Texas coast, feral
pigs eat a variety of items, including fruits, roots, mushrooms,
and invertebrates, depending on the season. The major foods in
spring are herbage, roots, invertebrates, and vertebrates. Fruit,
invertebrates, and herbage are most common in fall and winter
diets. Herbage eaten by feral pigs includes water hyssop, pennywort,
frog fruit, spadeleaf, onion, and various grasses while important
roots used for food include bulrush, cattail, flatsedges, and
spikesedges. Fruits and seeds such as grapes, acorns, and cultivated
sorghum are important, and animal matter ingested by feral pigs
includes earthworms, marsh fly larvae, leopard frogs, snakes,
and rodents.
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Feral pigs can have detectable influences
on wildlife and plant communities as well as domestic crops
and livestock. Extensive disturbance of vegetation and soil
occurs as a result of their rooting habits. The disturbed area
may cause a shift in plant succession on the immediate site.
Feral pigs also compete, to some degree, with several species
of wildlife for certain foods, particularly mast.
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Feral pigs generally
breed year round; litters range from one to seven, averaging two
per sow. An average of one to three suckling pigs usually accompanies
brood sows. The heat period is only about 48 hours in duration
and the average gestation period is 115 days.
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Role of p53 gene in sus scrofa
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The tumor
suppressor protein p53 plays an important role in the cell-cycle
G 1 and G 2 checkpoints. In response to DNA damage, p53 can induce
the transcription of p21, which inhibits the activation of various
G 1 cyclin/cyclin-dependent kinase complexes.It is not known whether
p53 plays a role in the initial migration of vascular smooth muscle
cells from the arterial tunica media (mVSMCs).
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| In this study,
we have investigated whether mVSMC migration from healthy tunica
media of young pigs and proliferation are regulated by p53. After
6 hours of incubation in mitogen-rich medium, explanted porcine
tunica media tissue showed complete downregulation of p53 protein
and p53 mRNA. The blockage of gene activity was not due to DNA methylation
at the 5' control region of the gene. The mVSMC outgrowth did not
show p53 expression. Mitogen-depletion of cultured p53 - /mVSMCs
did not restore p53 expression. Incubation of explanted porcine
tunica media tissue in mitogen-deprived medium increased p53 protein
content and blocked mVSMC outgrowth from the explant. As in p53-deficient
rodent cells, mVSMCs incubated with colcemid overrode the spindle-dependent
checkpoint, giving polyploidy and chromosomal pairing. UV-induced
DNA damage in mVSMCs incubated with mitogen-free medium induced
p53 expression and apoptotic cell death showing DNA nucleosomal
laddering. However, UV-irradiated mVSMCs incubated in mitogen-rich
medium did not express p53 and did not show cell death.In conclusion,
our results demonstrate that early mVSMC migration from the tunica
media requires mitogen-induced suppression of p53 that is highly
expressed in contractile mVSMCs residing in the healthy vessel wall.
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