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p53 Database
p53 Structures

Sus scrofa is the wild ancestor of the domestic pig. It is native in woodlands across much of Central Europe , the Mediterranean Region (including North Africa 's Atlas Mountains ) and much of Asia as far south as Indonesia , and has been widely introduced elsewhere. It is in the same Suidae biological family as the Warthog and Bushpig of Africa, the Pygmy Hog of northern India , Babirusa of Indonesia and others.

Description

Feral pigs in Texas are descended from introductions of European wild hogs for sporting purposes, and from escaped domestic swine that have established feral populations. European wild hogs have several distinguishing characteristics that set them apart from domestic or feral hogs. Among these are brown to blackish brown color, with grizzled guard hairs, a mane of hair (8-16 cm long) running dorsally from the neck to the rump, a straight heavily tufted tail, and ears covered with hair. Characteristics of feral hogs are varied, depending upon the breed of the ancestral stock. European wild hogs and feral hogs interbreed readily, with traits of European wild hogs apparently being dominant.

Habits

Good feral hog habitat in timbered areas consists of diverse forests with some openings. The presence of a good litter layer to support soil invertebrates and/or the presence of ground vegetation affording green forage, roots, and tubers is desirable. Hogs are also fond of marsh and grass-sedge flats in coastal areas, particularly if wild grapes are common. During hot summer months, "wallows," or depressions dug in the mud by feral hogs, are much in evidence near marshes or standing water, such as along roadside ditches.

On the Texas coast, feral pigs eat a variety of items, including fruits, roots, mushrooms, and invertebrates, depending on the season. The major foods in spring are herbage, roots, invertebrates, and vertebrates. Fruit, invertebrates, and herbage are most common in fall and winter diets. Herbage eaten by feral pigs includes water hyssop, pennywort, frog fruit, spadeleaf, onion, and various grasses while important roots used for food include bulrush, cattail, flatsedges, and spikesedges. Fruits and seeds such as grapes, acorns, and cultivated sorghum are important, and animal matter ingested by feral pigs includes earthworms, marsh fly larvae, leopard frogs, snakes, and rodents.

Feral pigs can have detectable influences on wildlife and plant communities as well as domestic crops and livestock. Extensive disturbance of vegetation and soil occurs as a result of their rooting habits. The disturbed area may cause a shift in plant succession on the immediate site. Feral pigs also compete, to some degree, with several species of wildlife for certain foods, particularly mast.

Feral pigs generally breed year round; litters range from one to seven, averaging two per sow. An average of one to three suckling pigs usually accompanies brood sows. The heat period is only about 48 hours in duration and the average gestation period is 115 days.

Role of p53 gene in sus scrofa

The tumor suppressor protein p53 plays an important role in the cell-cycle G 1 and G 2 checkpoints. In response to DNA damage, p53 can induce the transcription of p21, which inhibits the activation of various G 1 cyclin/cyclin-dependent kinase complexes.It is not known whether p53 plays a role in the initial migration of vascular smooth muscle cells from the arterial tunica media (mVSMCs).

In this study, we have investigated whether mVSMC migration from healthy tunica media of young pigs and proliferation are regulated by p53. After 6 hours of incubation in mitogen-rich medium, explanted porcine tunica media tissue showed complete downregulation of p53 protein and p53 mRNA. The blockage of gene activity was not due to DNA methylation at the 5' control region of the gene. The mVSMC outgrowth did not show p53 expression. Mitogen-depletion of cultured p53 - /mVSMCs did not restore p53 expression. Incubation of explanted porcine tunica media tissue in mitogen-deprived medium increased p53 protein content and blocked mVSMC outgrowth from the explant. As in p53-deficient rodent cells, mVSMCs incubated with colcemid overrode the spindle-dependent checkpoint, giving polyploidy and chromosomal pairing. UV-induced DNA damage in mVSMCs incubated with mitogen-free medium induced p53 expression and apoptotic cell death showing DNA nucleosomal laddering. However, UV-irradiated mVSMCs incubated in mitogen-rich medium did not express p53 and did not show cell death.In conclusion, our results demonstrate that early mVSMC migration from the tunica media requires mitogen-induced suppression of p53 that is highly expressed in contractile mVSMCs residing in the healthy vessel wall.