Comparative Genome Analysis and Development of Database System
for Apple Mosaic Virus; RNA1, RNA2, RNA3
and
Apple Chlorotic Leaf Spot Virus strain P863

Home Page
Apple Mosaic Virus
Apple Chlorotic leaf Spot Virus
Genomes & Genes
Proteins & Domains
Identified ORFs
Results & Discussion
Acknowledgement
Contact us

Project Developed by;
Pankaj Kr. Agarwal
B.E. (Biotechnology)
Dept. of Biotechnology
SEC Sikar, Rajasthan.
INDIA

email:
pankajbt@gmail.com

This Database system provides the background informations and results of Comparative Genome Analysis of Apple Mosaic Virus strains; RNA1, RNA2, RNA3 and Apple Leaf Chlorotic Virus strain P863.

The complete nucleotide sequences of apple mosaic virus RNA 1, 2 and 3 apple chlorotic leaf spot viruses strain P863 have been characterized. Apple mosaic virus RNA 1 is 3476 nucleotides in length and encodes a single large open reading frame (ORF), whereas apple mosaic virus RNA 2 is 2979 nucleotides in length and also encodes a single ORF and apple mosaic virus RNA3 is 2056 nucleotides in length and encodes two ORFs. While apple chlorotic leaf spot viruses strain P863 is 7555 nucleotide in length and encodes three ORFs.

The amino acids sequences encoded by apple mosaic virus RNA1, RNA 2 and RNA 3 show similarity to the amino acids sequences encoded by apple chlorotic leaf spot viruses strain P863. Apple mosaic virus RNA 3 and apple chlorotic leaf spot viruses strain P863 have close relationship in the movement protein and coat protein, but not with the replicase of apple chlorotic leaf spot viruses, putative polymerase of RNA 2 and putative methyl transferase of RNA 1. This study is based on the comparative analysis of protein coding genes in these viruses to identify the actual functionality and phylogeny. We also identify some short ORF sequences in these viruses and report them with there putative functional annotations. Complied data can be freely access from http://www.quickmt.com/projects/apple_virus/analysis.htm

We start our analysis with the identification of ORF's in these organism's. The ORF search was performed by using the NCBI/ORF Finder.
Table: Showing the ORF's identified in these organisms

Organism Name
ORF Number's
Apple Mosaic Virus RNA1
20
Apple Mosaic Virus RNA2
9
Apple Mosaic Virus RNA3
14
Apple Leaf Chlorotic Virus strain P863
33

* Click ORFs Link for Detail Information of Identified ORF's
* Click Genomes & Genes Link for Detail Information of Genomes and Genes
* Click Proteins & Domains Link for Detail Information of Proteins and Domains
* Click Results Link for Detail of Results of Comparative Analysis and Phylogenetic Analysis


 
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