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Conclusion
India
is established as an agricultural country and the 65% economy is
based on the agriculture production. So, many government and private
research labs are working to find the new techniques to increase
the production and quality of crops and fruits plants. The main
problem is that all the labs working on the identification for the
new techniques for the better production but there are only few
labs which are working for curing plants disease. For this, it is
necessary to mark out the infectious organism at molecular level.
It is a well know fact that wet-lab analysis are essential for this
kind of research. Here, we try to apply the computational research
or bioinformatics approaches to analysis the four viruses strains
named Apple Mosaic virus; RNA1, RNA2, RNA3 and Apple Chlorotic Leaf
Spot Virus P863, which infects the Apple plant leafs. Due to this
infection the production of apples may suffer. The aim of this study
is to provide the molecular level information about Apple Mosaic
virus; RNA1, RNA2, RNA3 and Apple Chlorotic Leaf Spot Virus P863.
We identified ApMV RNA 1 codes 20 ORFs, ApMV RNA 2 codes 9 ORFs,
ApMV RNA 3 codes 14 ORFs and ACLSV P863 codes 33 ORFs. Some of them
codes for the proteins, but we identify that both ApMV RNA 3 and
ACLSV P863 codes for the movement and coat proteins. Then, we focus
only on these two proteins to identify the similarity of these two
at sequence and structural level. As we thought that being the same
proteins they would be similar at functional and structure level,
after analysis we found them differ to each other. We were also
not capable to identify the exact 3D structures of these proteins
by the computational structure modeling techniques but we tried
to draw that. So, the final result is that, to analyze the mode
of infection and develop an efficient drug against these viral infections
we should treat them individually. We hope this information helps
agriculture research to develop the new insights for the further
research on the genes and protein involved in apple leaf diseases
caused by Apple Mosaic Virus and Apple Chlorotic Leaf Spot Virus.
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